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Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis

Nobre Evangelista, Andre Franciele Dos Santos, Flavia de Oliveira Martins, Lorena Pacheco Peixoto Gaiad, Thais Dias Machado, Alex Sander Rocha-Vieira, Etel Costa, Karine Beatriz Santos, Ana Paula Oliveira, Murilo Xavier

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DOI/PMID/Link: 10.1007/s10103-020-03155-3

Abstract

The aim of the present study was to investigate the effects of photobiomodulation (PBM) therapy on the expression of heat shock protein 70 (HSP70) and tissue repair in an experimental model of collagenase-induced Achilles tendinitis. Thirty Wistar rats (aged 12 weeks) were randomly distributed among control group (n = 8), tendinitis group (n = 11), and LED group (n = 11). Tendinitis was induced in the tendinitis and LED groups through a peritendinous injection of collagenase (100 μl). The LED group animals received the first irradiation 1 h after injury. A 630 ± 20 nm, 300-mW continuous wave light-emitting diode (LED), spot size 1 cm2, was placed in contact with the skin. One point over the tendon was irradiated for 30 s, delivering 9 J (9 J/cm2). LED irradiation was performed once daily for 7 days, with the total energy delivered being 63 J. The tendons were surgically removed and expression of the HSP70 protein was calculated using semi-quantitative analyses of immunohistochemistry (HSCORE). Number of fibroblasts and amount of collagen were measured using histological and histochemical analyses. An increase in the mean HSCORE for HSP70, in the number of fibroblasts, and in the amount of collagen were found in the LED group compared with those in the tendinitis and control group (P ≤ 0.05). PBM therapy increased the expression of the HSP70, number of fibroblasts, and amount of collagen in the acute Achilles tendinitis in rats.
Year Published 2021
Country Brazil
Rank Positive
Journal Lasers in Medical Science
Primary Topic Tendon
Secondary Topic Achilles Tendinitis
Tertiary Topic Tissue Regeneration
Model Rat
Wavelength (nm)
Complement/Comparison