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Photo biostimulatory effect of low dose photodynamic therapy on human mesenchymal stem cells

Fekrazad, Reza Asefi, Sohrab Khorsandi, Khatereh Nejatifard, Marzieh

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DOI/PMID/Link: 10.1016/j.pdpdt.2020.101886

Abstract

Background: Tissue engineering is one treatment to regenerate bone . Stem cell proliferation or differentiation can be stimulated by adjunctive approaches like photobiomodulation. Some studies suggested that, photodynamic therapy with low concentration of photosensitizers can stimulate cell differentiation as a photobiomodulation approach. Methods: Human bone marrow mesenchymal stem cell was isolated and then cultured in sterile medium. Two photosensitizer drugs as 5- aminolevulenic acid (1 mM) (5-ALA) and Methylene blue (1μM) (MB) were used in incubation culture media. In order to activate the photosensitizers, 630 and 660 nm wavelengths were irradiated with 1 J/cm2 energy density, respectively. Cell viability was assessed using MTT assay before and after laser irradiation, and also Alizarin red histologic test was used for calcium nodule formation. Results: performing the MTT test before irradiation showed that, the optimum concentrations were 1 mM for 5-ALA and 1μM for MB that were optimized. After laser irradiation, ALA group showed no osseous differentiation. In contrast, there was a significant calcium nodule formation in MB group compared with the control one. Conclusions: Photodynamic therapy with low photosensitizer concentration and low doses of laser energy density may improve osteogenic differentiation. Accordingly, MB had stimulatory effect on bone marrow derived mesenchymal stem cells. However, 5-ALA did not show this effect.
Year Published 2020
Country Iran
Rank Positive
Journal Photodiagnosis and Photodynamic Therapy
Primary Topic Bone
Secondary Topic Bone Regeneration
Tertiary Topic Stem Cell Therapy
Model Cell Culture
Wavelength (nm)
Complement/Comparison Aminolevulinic Acid, Methylene Blue