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Newly formulated 5% 5-aminolevulinic acid photodynamic therapy on Candida albicans

Greco, Giuseppe De Piazza, Simone Chan, Jiemei Zotti, Mirca Hanna, Reem Gheno, Ezio Zekiy, Angelina O. Pasquale, Claudio De Angelis, Nicola Amaroli, Andrea

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DOI/PMID/Link: 10.1016/j.pdpdt.2019.10.010

Abstract

Background: A large number of systemic diseases can be linked to oral candida pathogenicity. The global trend of invasive candidiasis has increased progressively and is often accentuated by increasing Candida albicans resistance to the most common antifungal medications. Photodynamic therapy (PDT) is a promising therapeutic approach for oral microbial infections. A new formulation of 5-aminolevulinic acid (5%ALA) in a thermosetting gel (t) (5%ALA-PTt) was patented and recently has become available on the market. However, its antimicrobial properties, whether mediated or not by PDT, are not yet known. In this work we characterised them. Methods: We isolated a strain of C. albicans from plaques on the oral mucus membrane of an infected patient. Colonies of this strain were exposed for 1 24 h, to 5%ALA-PTt, 5%ALA-PTt buffered to pH 6.5 (the pH of the oral mucosa) (5%ALA-PTtb) or not exposed (control). The 1 h-exposed samples were also irradiated at a wavelength of 630 nm with 0.14 watts (W) and 0.37 W/cm2 for 7 min at a distance of <1 mm. Results and conclusion: The 5% ALA-PTt preparation was shown to be effective in reducing the growth of biofilm and inoculum of C. albicans. This effect seems to be linked to the intrinsic characteristics of 5%ALA-TPt, such acidic pH and the induction of free radical production. This outcome was significantly enhanced by the effect of PDT at relatively short incubation and irradiation times, which resulted in growth inhibition of both treated biofilm and inoculum by ∼80% and ∼95%, respectively. Keywords: Candidiasis; Laser therapy; Mucositis; Oral infection; Oral thrush; Photodynamic Therapy; Stomatitis.
Year Published 2020
Country Italy
Rank Positive
Journal Photodiagnosis and Photodynamic Therapy
Primary Topic Mouth
Secondary Topic Oral Candidiasis
Tertiary Topic Pathogenic Bacterial Load
Model Cell Culture
Wavelength (nm)
Complement/Comparison Aminolevulinic Acid