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EFFECTIVENESS OF THE PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT HUMAN CELLS ІN A RESTING STATE

Lavrenchuck, G.Y. Talko, V.V. Chernyshov, A.V.

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DOI/PMID/Link: 10.33145/2304-8336-2023-28-206-215

Abstract

Objective: to investigate the structural and morphofunctional changes in test system of malignant (cell line A-549) human cells in a resting state exposed to X-rays in the presence of gadolinium-containing photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Photolon» photosensitizer. Methods: Passaged malignant human cell culture technology, X-ray and red light exposure, cytological and statistical methods. Results: X-ray exposure at a dose of 10.0 Gy in the presence of photon capture agent «Dotavist» (at a 100 μg/ml nutrient medium concentration) led to death of 75-83 % of malignant cells in a resting state on the 6-8th day of cultivation. Photodynamic exposure (630 nm wavelength red light) in the presence of «Photolon» photosensitizer (200 μg/ml concentration) resulted in death of 69-73 % of malignant cells, respectively. Combination of the photon-capturing technology and photodynamic exposure resulted in death of 90 % of the malignant cells in a phase of steady-state growth on the 8th day of cultivation. Conclusion: Combination of the photon capture technology (X-ray exposure with gadolinium-containing photoncapture agent «Dotavist» in cytotoxic concentration) and photodynamic exposure in the presence of «Photolon» photosensitizer increased devitalization effectiveness of human non-small cell lung cancer cells (A-549 cell line) being in a steady-state growth phase up to 90 %. Ten percent of cells resistant to the applied technologies retained their proliferative potential, evident as changes in their morphology, genotype and adhesiveness during further cultivation. Keywords: X-ray irradiation; culture of human malignant cells; photon capture agent; photosensitizer; red light.
Year Published 2023
Country Ukraine
Rank Positive
Journal Problemy Radiatsiinoi Medystyny ta Radiobiolohii
Primary Topic Lung
Secondary Topic Cancer
Tertiary Topic Photodynamic Therapy
Model Cell Culture
Wavelength (nm)
Complement/Comparison Photolon Photosensitizer